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anti gdf11 primary antibody  (R&D Systems)


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    R&D Systems anti gdf11 primary antibody
    Anti Gdf11 Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gdf11/Recombinant+Human%2FMouse%2FRat+GDF-11%2FBMP-11+Protein/pm41637825-94-14-19
    Average 93 stars, based on 28 article reviews
    anti gdf11 primary antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Incubation:

    Article Title: Endogenous GDF11 regulates odontogenic differentiation of dental pulp stem cells
    Article Snippet: .. The frozen sections and the cellular coverslips were incubated overnight with appropriate primary antibodies anti‐GDF11 (1:500, #MAB 19581, R&D System) and anti‐β‐catenin (1:500, #51067‐2‐AP, Proteintech), respectively, at 4°C. ..

    Article Title: Growth differentiation factor 11 is involved in isoproterenol-induced heart failure
    Article Snippet: .. Proteins (50 μg) were separated via 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and blocked using 5% non-fat milk at room temperature for 1 h. Subsequently, the membranes were incubated overnight at 4°C with the following primary antibodies: Anti-B-cell lymphoma 2 (Bcl-2; 1:1,000; cat. no. 3498; Cell Signaling Technology, Inc., Danvers, MA, USA); anti-Bcl-2-associated X protein (Bax; 1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.); anti-nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2; 1:1,000; cat. no. 19013-1; ProteinTech Group, Inc., Chicago, IL, USA); anti-Nox4 (1:1,000; cat. no. 14347-1; ProteinTech Group, Inc.); anti-GDF11 (1:1,000; cat. no. AF1958; R&D Systems, Inc.) and anti-GAPDH as an internal control (1:2,000; cat. no. 10494-1; ProteinTech Group, Inc.). .. Following three washes in TBS-Tween 20 (0.5 ml/l), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:1,000; cat. no. A0208; Beyotime Institute of Biotechnology) at room temperature for 1 h. Target bands were detected using the SuperSignal West Pico Chemiluminescent Substrate (Pierce; Thermo Fisher Scientific, Inc.), and band intensity was quantified using ImageJ 1.48i software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Growth differentiation factor 11 is involved in isoproterenol‑induced heart failure.
    Article Snippet: .. Proteins (50 μg) were separated via 10% SDS‐PAGE, transferred to polyvinylidene difluoride membranes, and blocked using 5% non‐fat milk at room temperature for 1 h. Subsequently, the membranes were incubated overnight at 4 ̊C with the following primary antibodies: Anti-B-cell lymphoma 2 (Bcl-2; 1:1,000; cat. no. 3498; Cell Signaling Technology, Inc., Danvers, MA, USA); anti-Bcl-2-associated X protein (Bax; 1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.); anti-nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2; 1:1,000; cat. no. 19013-1; ProteinTech Group, Inc., Chicago, IL, USA); anti-Nox4 (1:1,000; cat. no. 14347-1; ProteinTech Group, Inc.); anti-GDF11 (1:1,000; cat. no. AF1958; R&D Systems, Inc.) and anti-GAPDH as an internal control (1:2,000; cat. no. 10494-1; ProteinTech Group, Inc.). .. Following three washes in TBS-Tween 20 (0.5 ml/l), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:1,000; cat. no. A0208; Beyotime Institute of Biotechnology) at room temperature for 1 h. Target bands were detected using the SuperSignal West Pico Chemiluminescent Substrate (Pierce; Thermo Fisher Scientific, Inc.), and band intensity was quantified using ImageJ 1.48i software (National Institutes of Health, Bethesda, MD, USA).

    Staining:

    Article Title: Mutations in GDF11 and the extracellular antagonist, Follistatin, as a likely cause of Mendelian forms of orofacial clefting in humans
    Article Snippet: .. Sections were then stained with the following primary antibodies: anti-FST [H114] (1:100, Santa Cruz #sc30194) and anti-GDF11 (1:100, R&D Systems #MAB19581) followed by a biotinylated secondary antibody and ABC-HRP of the appropriate specificity as previously described ( Tamasas & Cox, 2017 ). ..

    Article Title: Mutations in GDF11 and the extracellular antagonist, Follistatin, as a likely cause of Mendelian forms of orofacial clefting in humans.
    Article Snippet: .. Sections were then stained with the following primary antibodies: anti‐FST [H114] (1:100, Santa Cruz #sc30194) and anti‐GDF11 (1:100, R&D Systems #MAB19581) followed by a biotinylated secondary antibody and ABC‐HRP of the appropriate specificity as previously described (Tamasas & Cox, 2017). ..

    Blocking Assay:

    Article Title: Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy
    Article Snippet: Equal amounts of proteins were subjected to sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and then transferred to polyvinyldifluoride membranes (IPVH00010; Merck Millipore). .. After blocking, the membranes were probed with the following primary antibodies: anti‐GDF11 (1:1000, MAB19581; R&D Systems), anti‐p16 (1:1000), anti‐p21 (1:500; Proteintech, 10355‐1‐AP), anti‐proliferating cell nuclear antigen (PCNA, 1:1000, 2586; Cell Signaling Technology), anti‐autophagy‐related (Atg) 16L1 (1:1000, AP18176; Abgent), anti‐LC3B (1:1000), anti‐p62 (1:1000), anti‐Atg5 (1:500, 12994; Cell Signaling Technology), anti‐cathepsin B antibody (1:500), anti‐ATPase H+ transporting V1 subunit a (ATP6V1a, 1:1000, ab19270; Abcam), anti‐ATP6V1b2 (1:1000, ab73404; Abcam), anti‐TFEB (1:1000, H00007942‐M01; Novus Biologicals), anti‐extracellular signal‐regulated protein kinase 1/2 (ERK1/2, 1:1000, 9102; Cell Signaling Technology), anti‐phos‐ERK1/2 (Thr202/Tyr204) (1:1000, 9101; Cell Signaling Technology), anti‐70 kDa ribosomal protein S6 kinase 1(p70S6K1,1:2000, 2708; Cell Signaling Technology), anti‐phos‐p70S6K1 (T389) (1:1000, 9234; Cell Signaling Technology), anti‐eukaryotic initiation factor 4E‐binding protein 1 (4E‐BP1, 1:1000, 9452; Cell Signaling Technology), anti‐phos‐4E‐BP1 (Thr37/46) (1:1000, 2855; Cell Signaling Technology), anti‐AKT(1:1000, 9272; Cell Signaling Technology), anti‐phos‐AKT(Ser473) (1:1000, 4060; Cell Signaling Technology), anti‐Histone H3(1:1000, ab1791; Abcam), and anti‐GAPDH antibody (1:20,000; G9545, Sigma‐Aldrich). .. Western blots were acquired with the Kodak Image Station (Carestream Health Inc), and bands were quantified by densitometry using Image J software normalizing against GAPDH or Histone H3.

    Control:

    Article Title: Growth differentiation factor 11 is involved in isoproterenol-induced heart failure
    Article Snippet: .. Proteins (50 μg) were separated via 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and blocked using 5% non-fat milk at room temperature for 1 h. Subsequently, the membranes were incubated overnight at 4°C with the following primary antibodies: Anti-B-cell lymphoma 2 (Bcl-2; 1:1,000; cat. no. 3498; Cell Signaling Technology, Inc., Danvers, MA, USA); anti-Bcl-2-associated X protein (Bax; 1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.); anti-nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2; 1:1,000; cat. no. 19013-1; ProteinTech Group, Inc., Chicago, IL, USA); anti-Nox4 (1:1,000; cat. no. 14347-1; ProteinTech Group, Inc.); anti-GDF11 (1:1,000; cat. no. AF1958; R&D Systems, Inc.) and anti-GAPDH as an internal control (1:2,000; cat. no. 10494-1; ProteinTech Group, Inc.). .. Following three washes in TBS-Tween 20 (0.5 ml/l), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:1,000; cat. no. A0208; Beyotime Institute of Biotechnology) at room temperature for 1 h. Target bands were detected using the SuperSignal West Pico Chemiluminescent Substrate (Pierce; Thermo Fisher Scientific, Inc.), and band intensity was quantified using ImageJ 1.48i software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Growth differentiation factor 11 is involved in isoproterenol‑induced heart failure.
    Article Snippet: .. Proteins (50 μg) were separated via 10% SDS‐PAGE, transferred to polyvinylidene difluoride membranes, and blocked using 5% non‐fat milk at room temperature for 1 h. Subsequently, the membranes were incubated overnight at 4 ̊C with the following primary antibodies: Anti-B-cell lymphoma 2 (Bcl-2; 1:1,000; cat. no. 3498; Cell Signaling Technology, Inc., Danvers, MA, USA); anti-Bcl-2-associated X protein (Bax; 1:1,000; cat. no. 5023; Cell Signaling Technology, Inc.); anti-nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2; 1:1,000; cat. no. 19013-1; ProteinTech Group, Inc., Chicago, IL, USA); anti-Nox4 (1:1,000; cat. no. 14347-1; ProteinTech Group, Inc.); anti-GDF11 (1:1,000; cat. no. AF1958; R&D Systems, Inc.) and anti-GAPDH as an internal control (1:2,000; cat. no. 10494-1; ProteinTech Group, Inc.). .. Following three washes in TBS-Tween 20 (0.5 ml/l), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:1,000; cat. no. A0208; Beyotime Institute of Biotechnology) at room temperature for 1 h. Target bands were detected using the SuperSignal West Pico Chemiluminescent Substrate (Pierce; Thermo Fisher Scientific, Inc.), and band intensity was quantified using ImageJ 1.48i software (National Institutes of Health, Bethesda, MD, USA).



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    Image Search Results


    GDF11 activates TGF-β signaling (Referring to Sheen et al., 2013, PMID: 24244818 ).

    Journal: Heliyon

    Article Title: Rejuvenation of young blood on aging organs: Effects, circulating factors, and mechanisms

    doi: 10.1016/j.heliyon.2024.e32652

    Figure Lengend Snippet: GDF11 activates TGF-β signaling (Referring to Sheen et al., 2013, PMID: 24244818 ).

    Article Snippet: They have found that this immunoglobulin light chain weighs approximately 25 kDa and can also be detected by the Abcam anti-GDF11 antibody [ ].

    Techniques: